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In 1996, California passed Proposition 215, making it the first of many states to ultimately legalize medical cannabis; as of January 2020, an additional 32 states and the District of Columbia have also made medical cannabis legal. Additionally, recreational use of cannabis is now legal in 11 states and is decriminalized in many others. Cannabis legalization and decriminalization have made cannabidiol (“CBD”, a non-psychoactive compound found in cannabis), and tetrahydrocannabinol(“THC”, the chemical responsible for most of cannabis’ mind-altering effects), available to both recreational users and patients seeking treatment for such health issues as arthritis, anxiety, inflammation, seizure disorders, and nausea.
Since California’s groundbreaking move in 1996, medical and recreational cannabis has become a significant and rapidly growing industry. According to DC-based cannabis researcher, New Frontier Data, legal cannabis sales in the U.S. are expected to reach $30 billion annually by 2025. The industry growth has led to a substantial increase in grow rooms, medical dispensaries and other retail outlets, and extraction facilities.
Extraction
Extraction is a process by which desired chemical compounds are extracted and separated from the cannabis plant. Extraction strips the plant of essential oils, including CBD, THC, and terpenes (aromatic oils that give cannabis plants their distinctive scents). The extracted oils can be utilized in vape pens, edibles, capsules, tinctures, and topical solutions. Based on the end product, various techniques can be used for extracting the oils, including carbon dioxide (CO2) extraction and hydrocarbon solvent extraction (using solvents such as butane or propane).
Carbon Dioxide Extraction
Carbon dioxide, high pressure, and heat can be combined to create a “supercritical fluid” that extracts cannabis components from the plant. The CO2 extraction method generally produces high yields with relatively little waste. Temperatures and pressures can be adjusted to create multiple products including vaporizer oils; dabbing concentrates such as so-called waxes, crumble, shatters, and saps; and distillates (cannabis extracts that have been further purified and processed to separate and isolate the various cannabinoids, which include CBD and THC). Because CO2 evaporates on its own, many in the medical products and food and beverage industries find the CO2 extraction method appealing, since no residual carbon dioxide remains in the final manufactured product.
Hydrocarbon Solvents Extraction
Hydrocarbon extraction typically uses organic solvents such as butane and propane to separate essential oils from the plant material. The use of hydrocarbons for extraction is popular owing, in large part, to the relatively low overhead costs, efficiency (including the wide variety of products that can be created from a single extraction, without the need for further refinement), and high product quality associated with this technique. For instance, the low boiling point of butane, and even lower boiling point of propane, allow extractors to remove the desired compounds without risking evaporation of, or damage to, the delicate and heat-sensitive cannabinoids and terpenes. Moreover, their low boiling points makes it relatively easy to purge any residual butane or propane at the end of the extraction process, leaving behind only a relatively pure product.
Oxygen Monitors Can Protect Extractors and Their Employees
 While CO2 and hydrocarbon solvents are important techniques for extracting essential oils from cannabis plants use of these gases is not without risk, since extraction facility personnel and property are exposed to potential leaks from gas supply lines and storage containers.
Carbon dioxide is an oxygen-depleting gas that is both odorless and colorless. As such, absent appropriate monitoring to detect that a leak has occurred, extraction employees could become dizzy, lose consciousness, and even suffocate from breathing oxygen-deficient air. Hydrocarbons such as butane and propane also deplete oxygen and, they are flammable and explosive as well.
Proper gas detection equipment should be placed where the cannabis extraction process takes place, as well as in CO2 and hydrocarbon storage rooms, and in any other site where CO2, butane, and propane may be expected to accumulate. The gas detection equipment should include the capacity to activate visual and audible alarms, stopping the flow of gas and turning on the ventilation system.
PureAire Monitors
PureAire Monitoring Systems has safety monitors to meet the needs of cannabis extractors, whether they use CO2 or hydrocarbon solvents.
For facilities using carbon dioxide to extract their products, PureAire’s line of dual oxygen/carbon dioxide monitors offer thorough air monitoring, with no time-consuming maintenance or calibration required. The O2/CO2 monitor comes with user-adjustable alarm setpoints for both oxygen and carbon dioxide. The monitor is built with zirconium oxide sensor cells and non-dispersive infrared sensor (NDIR)cells, to ensure longevity.PureAire’s O2/CO2 monitors can last, trouble-free, for over 10 years under normal operating conditions.

Extractors utilizing hydrocarbon solvents, such as butane or propane, rely on PureAire’s LEL, explosion-proof, combustible gas monitors. The monitor is housed in a NEMA 4 enclosure specifically designed to prevent an explosion. The durable, long-life LEL catalytic sensor will last 5+ years without needing to be replaced.
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PureAire monitors feature an easy to read screen, which displays current oxygen levels for at-a-glance observation by employees, who derive peace of mind from the monitor’s presence and reliable performance. In the event of a gas leak, or a drop in oxygen to an unsafe OSHA action level, PureAire’s monitors will set off alarms, complete with horns and flashing lights, alerting personnel to evacuate the area. At the same time, the monitors can be programmed to turn off the flow of gas (CO2, butane, or propane, as appropriate), and turn on the ventilation system.
In short, PureAire’s monitors enable cannabis extractors, in a cost-effective manner, to preserve both the quality of their products and the well-being of their employees.

Overview
3D printing (also known as “additive manufacturing”) affords manufacturers the ability to create custom parts that fit together perfectly.  Utilized for decades in the medical products and aerospace parts industries, 3D printing is increasingly being used in other industries as well, including the relatively recent advent of 3D printed metal auto parts.

 New and Replacement Auto Parts
Automakers have made use of 3D printing processes since the late 1980s, with the initial output comprised primarily of plastic parts.  Manufacturers such as Ford, BMW, Bugatti, Chrysler, Honda, Toyota, among others, have embraced 3D printing in their research and development efforts, including the production of working prototypes.  While the automobile industry is currently unable to mass produce an all 3D printed vehicle, carmakers are already producing 3D printed parts, with the eventual goal, as soon as is feasible, of more fully integrating 3D printed parts into the original manufacture of future generations of automobiles.

Availing themselves of 3D printing processes for producing auto parts allows manufacturers to generate parts that are lightweight (which can improve fuel efficiency) and customizable, and that can be created quickly, enhancing the lean manufacturing focus on just in time inventory.  Although plastic has traditionally been the material most often used in printing parts, as advances in additive manufacturing have been made, so too has the use of alternative materials.

For instance, in 2018, French luxury automaker Bugatti announced that it had developed a new 3D printed titanium brake caliper prototype which, it claimed, was the largest functional titanium component produced with a 3D printer.  DS Automobiles, Citroen premium brand, has created 3D titanium printed parts for the ignition elements, as well as 3D printed titanium door handles, to give their DS 3 Dark Side edition vehicle a sleek, high tech feel.

Gas Usage In 3D Printing Process
To prevent corrosion, and to keep out impurities that can negatively impact the final product, 3D printed parts must be produced in an environment made free of oxygen, typically by the use of argon (and sometimes nitrogen) within the building chamber. That creates a stable printing environment, prevents fire hazards by keeping combustible dust inert, and controls thermal stress in order to reduce deformities.

Oxygen Monitors Can Improve Safety in Additive Manufacturing Processes
​Dust from materials used in additive manufacturing, such as titanium, is, when exposed to oxygen, highly combustible and, therefore, requires monitoring to prevent possible explosions.Argon and nitrogen, while used in 3D printing for their oxygen depleting properties, require monitoring to ensure both the integrity of the finished part, and the safety of manufacturing personnel.

PureAire Monitors 

For quality control purposes, PureAire Monitoring Systems’ Air Check O2 0-1000ppm monitor has a remote sensor that can be placed directly within the printing build chamber, to continuously monitor the efficiency and purity of the O2 depleting gases (e.g. argon and nitrogen) used therein.
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Moreover, to ensure employee safety, PureAire’s Oxygen Deficiency Monitors should be placed anywhere argon and nitrogen supply lines and storage tanks are located. In the event of an argon or nitrogen leak, a drop in oxygen will cause the built-in horn to sound and the lights to flash, thereby alerting employees to evacuate the area.  PureAire’s Oxygen Deficiency Monitors measure oxygen 24/7, with no time-consuming maintenance required. PureAire’s monitors feature long-lasting zirconium sensors, which are designed to give accurate readings, without calibration, for up to 10 years.

Many industries use compressed gas to create products. While compressed gases such as nitrogen are low-cost, easy to use, and flexible in a range of industries, these gases have a hidden downside: They displace oxygen from the air, which puts your workers at risk of suffocation if there's a leak. A room oxygen monitor checks levels of oxygen and provides in-time alerts if there's a gas leak. Learn what a room oxygen monitor does, how it works, and who needs one. 

What Does an Oxygen Monitor Do? 

Inert gases, such as nitrogen, displace oxygen. Since these gases cannot be seen or smelled, facilities need a tool that's capable of detecting gas leaks. An oxygen monitor tracks levels of oxygen in a room and provides efficient notification if oxygen levels fall as the result of a gas leak. 

Oxygen monitors may be called O2 monitors or oxygen deficiency monitors. While these names are all synonymous, there are a few other terms you might hear that do not refer to this kind of oxygen monitor. 

In the medical and pharmaceutical industries, you may come across blood oxygen monitor, pulse oximetry, or oximeter products. These are totally different products than the oxygen deficiency monitor, and they will not protect against gas leaks. You'll find medical oximeters sold at pharmacies and online retailers, while oxygen deficiency monitors are sold online, through distributors, or directly from manufacturers like PureAire.  
Which Industries Use an Oxygen Monitor? 
Oxygen monitors are used by businesses in the following industries: 

• Food and beverage 
• OLED
• Semiconductor 
• Automotive 
• Pharmaceutical 
• Medical gas
• MRI 
• Cryotherapy and cryohealth
• Cryopreservation 
• Egg freezing 
• Research and development 

Businesses in these industries commonly use gases such as nitrogen in everyday operations. An oxygen deficiency monitor not only provides in-time notification of gas leaks but may be required by regulations. Failing to install an oxygen deficiency monitor could leave you out of compliance, which could lead to fines. 

How Does an Oxygen Monitor Work? 

An oxygen monitor works by using a sensor to check levels of oxygen. A digital display interface shows readouts in PPM, PPB, or percentage, so your workers can tell at a glance that everything is functioning properly. 

When levels of oxygen are at naturally occurring levels, the oxygen monitor stays silent. Employees can still check the readout for peace of mind. When something is wrong, an loud alarm goes off to provide your workers with instant notification of a safety threat. 

PureAire's line of oxygen monitors feature a unique zirconium sensor, which is designed to function for 10 years or more with no maintenance. Unlike other types of O2 monitors on the market, our oxygen monitor does not need regular maintenance or calibration. Your facility will save time and money when you choose PureAire products. 

PureAire's O2 monitor perform in a range of environments, including confined spaces, basements, and freezers. Capable of accurate readouts in temperatures as low as -40 C, our oxygen monitors never drift from barometric pressure shifts or thunderstorms. 
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Do you have questions about oxygen deficiency monitors? We're here to answer your questions. Chat with us online or call today: 888.788.8050.

While OSHA regulations require the use of an oxygen monitor anywhere that compressed gases or cryogenic liquids are used or stored indoors, the regulation does not provide sufficient detail for facilities on how to set up am oxygen monitor. Businesses want to comply with the regulations, but are left wondering what compliance looks like. At PureAire, we're often asked by our customers, "how many oxygen sensors should installed?" so we thought we'd provide clarification on where and how to mount oxygen monitors. 

Where an Oxygen Deficiency Monitor Should be Used

OSHA regulations require that oxygen deficiency monitors be placed in any room where compressed gases are used or stored. Storage areas are frequently outside or in confined spaces, such as basements or storage closets. 

When gas tanks are installed outside and the gas enters the facility by pipes, we recommend oxygen deficiency monitors be installed near the main gas connections, which is where the gas enters the facility. This might be near a machine, a food and beverage packaging dispensing machine, a 3D printer, or other tool. 

With respect to a confined space where dewars of gas are kept, the oxygen deficiency monitor should be installed directly in the storage area. PureAire's oxygen monitors are designed to function optimally in confined spaces, including cryogenic freezers, and are impervious to shifts in barometric pressure. As such, they take accurate readouts of oxygen levels in confined spaces, freezers, facilities, and other places. 

The oxygen monitors measure  5.12 inches wide by 4.5 inches high by 3.25 inches deep, and their small size means that they're quite easy to place about the facility, even if you need to place the O2 monitor in a tight confined space, such as a cryogenic freezer. 

Best Place to Mount an Oxygen Deficiency Monitor 

Best practice is to mount the oxygen deficiency monitor 3 to 5 feet off the ground, as well as 3 to 5 feet away from a gas cylinder. 

There are situations when the oxygen monitor should be placed further away. One common example is MRI rooms, where metal is prohibited due to the strength of the MRI magnet. In these circumstances, the oxygen deficiency monitor can be mounted outside of the room, and a plastic sample draw tube used to check oxygen levels inside the MRI room. 

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What is the Proper Spacing of Oxygen Monitors? 

This last question may be the trickiest question to answer. Nitrogen and other inert gases have no odor or color, so they cannot be seen. The difficulty here is that it's all but impossible to say where the gas will go if there is a leak. 

We recommend that you place one oxygen deficiency monitor every 400 to 600 square feet to be safe. This works out to every 20 to 30 feet in a large space. When you use this ratio to determine the right number and spacing of oxygen monitors for your facility, you'll be adequately covered just in case anything happens. Given the deadly consequences of a nitrogen leak, it's better to be safe than sorry. 

PureAire creates oxygen deficiency monitors that are capable of withstanding some of the toughest conditions. Oxygen deficiency monitors from PureAire are designed to operate in temperatures as low as -40 C up to 55 C. 

Oxygen deficiency monitors can last for 10 or more years with no calibration. The hardy zirconium sensor needs no calibration after installation, which means that setup couldn't be easier. 

PureAire's monitors are accurate to +/- 1 percent and come with two alarm levels, 18 percent and 19.5 percent. The integrated alarms provide sufficient notification for workers to evacuate the area. The LCD display is backlit so it's easy to read.

All PureAire O2 monitors come with a 3 year warranty. Wall mounting brackets and an optional plug-in wall power supply are included, so you can mount the unit upon receipt and protect your facility from dangerous gas leaks. 

To learn more about PureAire's oxygen deficiency monitors, visit www.pureairemonitoring.com. 

Scientists from MIT and Harvard University are placing their faith in a gene editing tool that may revolutionize the treatment of deadly diseases. CRISPR, which stands for Clustered Regularly Interspaced Short Palindromic Repeats, has the potential to unlock the next generation of treatments for conditions like cancer, ALS, or Alzheimer's. Learn how CRISPR is poised to change genome editing and biomedicine over the next few decades.
 
How Does CRISPR Work? 
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Bacteria within the body have their own innate intelligence -- the fascination with the microbiome being one example of this scientific principle.
 
Scientists observed that bacteria was able to fight infections by retaining a slice of DNA from invading viruses, so they could recognize if the virus returned and mount a faster defense. If the intruder returns, the body's natural CRISPR goes after it. Scientists were able to create their own CRISPR, which they can use to edit genes.
 
You may remember all genes contain chemical basis, referred to by the letters C, G, A, or T. A genetic typo creates markers for disease. Scientists can search for specific bad combinations using CRISPR -- for instance, the gene that would cause ALS -- and then slice out the faulty gene and replace it with something innocuous. By doing this before someone gets sick, the theory goes, CRISPR can save lives. 
 
Already, scientists are using CRISPR to breed mosquitos that cannot transmit malaria, an application that would save thousands of lives. Others are working to create a stronger rice plant that can withstand floods and drought caused by climate change.
 
There are a few examples that illustrate the power of CRISPR.

Scientists are still figuring out the true potential of this genome editing tool, however, there is great promise and great enthusiasm for CRISPR's potential from scientists across the globe. In the meantime, laboratory workers must preserve genes and tissue samples for vitality using a nitrogen freezer.

Keeping Tissue Safe in the Laboratory Setting

Nitrogen freezers maintain ultralow temperatures of -150 to -200 Celsius. When genetic material is frozen at such a low temperature, it goes to sleep. The material can be thawed and reanimated for use in the lab setting. Along with low temperatures, the key to maintaining the vitality of the tissue is a slow freeze and thaw. If cells were to freeze too quickly, their cell membranes would burst. The same holds true for thawing frozen tissue. Thus, nitrogen freezers are a mainstay of the lab setting because they provide a reliable, efficient way to keep genomic materials chilled until use.

Any time nitrogen is used, there is a risk of accident if the nitrogen leaks or spills. Nitrogen does not have a color, scent, or odor, which means lab workers wouldn't notice a leak -- although they might notice if, say, the freezer door did not fully close.
 
Like other inert gases, nitrogen displaces oxygen. If the nitrogen freezer were to leak, the laboratory could lose so much oxygen that workers would experience respiratory distress. To safeguard against a leak, laboratories must use an oxygen deficiency monitor.
 
An oxygen deficiency monitor tracks the level of oxygen in the lab through constant monitoring. Since nitrogen displaces oxygen, this monitor can detect a gas leak by noting falling levels of oxygen. A digital display indicates the current amount of oxygen in the room, providing assurance for lab staff that everything is working as it should. If oxygen falls to the critical threshold as defined by OSHA, an alarm goes off. Lab workers can exit the premises and wait for emergency personnel to respond.

PureAire creates robust oxygen monitors trusted within the scientific and biomedical communities. PureAire's oxygen deficiency monitors work in freezing temperatures and confined spaces, remain accurate despite barometric pressure shifts, and last 10 or more years without calibration. 
 
To learn more about PureAire's products, visit www.pureairemonitoring.com

sited sources:

https://www.cbsnews.com/news/crispr-the-gene-editing-tool-revolutionizing-biomedical-research/
 
https://www.thermofisher.com/us/en/home/references/gibco-cell-culture-basics/cell-culture-protocols/freezing-cells.html
 
keywords: CRISPR, gas, laboratory, nitrogen, O2 Monitor, Oxygen Monitor, oxygen monitors, pureaire, safety